麻豆精选

• PGCert in Learning & Teaching in HE, University 麻豆精选, 2012
• PhD in Biochemistry, University 麻豆精选, 2003
• BSc (Hons) in Biochemistry with Molecular and Cell Biology, University 麻豆精选, 1999

Prior to joining Chemical Engineering, Dr Overton was a postdoctoral researcher in the School of Biosciences at the University 麻豆精选, studying microbial physiology and gene regulation in both model organisms (Escherichia coli) and human pathogens (Neisseria gonorrhoeae听补苍诲听E. coli O157) in response to oxygen and reactive species using transcriptomic, proteomic and other molecular biology techniques. Using systems biology and other molecular approaches, he identified mechanisms for bacterial survival in adverse environments.聽

Research became focused on bioprocessing topics during a BBSRC-EPSRC Bioprocessing Research Industry Club (BRIC) grant in collaboration with GSK, studying the production of difficult recombinant proteins in聽E. coli. Dr Overton was initially postdoctoral researcher on this project, and moved to a Co-I role upon taking up his position in the School of Chemical Engineering.聽

He has since built a research group focused on three main areas: production of high-value products using microbial fermentation; bacterial biofilms and how to use them to produce useful products or to remove them from where they are not desired; and how microbes can be eliminated from various processing streams. Research areas are further detailed below.

Dr Overton is director of MSc programmes in the School of Chemical Engineering and chairs the School MSc and EngD board of examiners. He is a member of the University Advisory Group on Biological Hazards and is School GMO safety officer and a member of the School safety committee. He is a member of BBSRC research committee D, reviews for research councils (BBSRC, EPSRC, MRC) and other funding bodies (Carnegie Trust of Edinburgh, NSF, National Biofilms Innovation Centre) and journals.

• MEng/BEng Chemical Engineering
• MSc Advanced Chemical Engineering
• EngD Formulation Engineering

Teaching topics include basic biology and molecular biology, systems and synthetic biology, genomic technologies, fermentation and cell culture and analysis of microbial physiology. Dr Overton also supervises fermentation practicals at laboratory and pilot scale (~100 litres).

We are interested in using information about the molecular microbiology and physiology of bacteria to develop processes. We focus on three main areas:

- Development of bioprocesses that generate high-value products (for example recombinant protein biopharmaceuticals, polymers, small molecules);
- Understanding biofilms and they ways in which their structure and function are regulated and might be modulated; and
- Developing processes that are designed to eliminate contaminating bacteria from product streams (for example foods and waste streams).

Research is split into three main themes. Throughout each stream we are interested in applying microbial physiology to solve real-world problems and optimise processes. We work closely with industry to do this.

Fermentation for useful products

Microbes are used for the production of many high-value products in fermentation processes. We investigate how these processes might be improved and optimised, to increase yields, shorten development times and accelerate innovation in bioproduction. Research has focused on a variety of product types.

• Recombinant proteins for biopharmaceutical use, manufactured in E. coli as a host. Research focuses on hard-to-manufacture proteins, stress minimisation, fermentation intensification, analysis of physiology in real time and transport of proteins to the periplasm (funded by BBSRC / EPSRC / InnovateUK KTP and in collaboration with industry).聽

• Biopolymers for replacement of petrochemically-derived plastics, made in C. necator. We are interested in improving polymer properties, measuring gene regulation during processes, and improving extraction of biopolymers using green approaches (funded by EU FP7 / BBSRC PhD studentship and in collaboration with industry).聽

• Magnetic nanoparticles are naturally generated by magnetotactic bacteria and allow them to sense the earth鈥檚 magnetic field. We are interested in engineering and manufacturing these particles for use in biotechnology and bioprocessing applications (funded by ERA-IB / BBSRC and in collaboration with industry).

Bacterial biofilms

Bacteria form biofilms, communities of cells immobilised onto surfaces by secreted polymeric substances, in many settings. Biofilms are typically tough and resistant to both physical removal and chemical treatment. This causes a problem in clinical and industrial settings, but we are also interested in how biofilms can be used as tough biocatalysts for production of fine chemicals. Within this area we investigate several approaches to understand how biofilms form and how we can interact with them.

• We demonstrated that E. coli biofilms are tough and efficient biocatalysts for the production of pharmaceutical precursor molecules. We are interested in implementing biofilms as a platform for biocatalysis (funded by BBSRC).聽

• There is growing evidence that mechanosensing is an important part of biofilm formation. We are studying how mechanosensing influences the formation and development of E. coli biofilms (funded by BBSRC PhD studentship).聽

• The development of E. coli biofilms also relies upon sensing of physicochemical cues; understanding how these impact on biofilm formation and development is essential to understanding and preventing biofilm formation.聽

• In collaboration with Procter & Gamble we are working on biofilms in industrial settings, how to prevent their formation and how to remove biofilms that have already formed (funded by EPSRC CDT scheme).聽

• We are interested in novel approaches to biofilm formation, including using polymers to nucleate and drive biofilm generation (funded by BBSRC PhD studentship).

Microbes in processing streams 聽聽

Microbes are ubiquitous in nature, but it is essential that they are removed from the processing streams for a variety of products such as food and fast-moving consumer goods. We are interested in developing processes that remove microbes from these settings.

• In collaboration with Colgate-Palmolive we are studying production of formulated products to improve robustness of formulations (funded by EPSRC CDT scheme).

• Dairy processing requires removal of pathogens from milk to ensure product safety, but generates large quantities of waste materials. We developed methods to process waste streams to generate added value (funded by InnovateUK and in collaboration with industry).

• Member of the executive board
• Co-chair of the European Federation of Biotechnology
• Organiser of Applied Synthetic Biology in Europe conference series
• Editorial board member for Biotechnology Letters
• Refereeing for BMC Microbiology, FEMS Microbiology Letters, DNA Sequence, Powder Technology and the MRC and National Science Foundation
• Peer review for FEBS Letters, Biotechnology Letters, BMC Microbiology, BMC Research Notes, FEMS Microbiology Letters, Powder Technology and DNA Sequence.
• Grant application review for BBSRC, MRC and the National Science Foundation